How to Reconstitute Peptides
Preparation:
- Wash hands thoroughly and clean your workspace.
- Remove caps from both the peptide and bacteriostatic water vials.
- Wipe the vial tops with alcohol pads and allow them to dry.
Draw Solvent:
- Insert the needle into the bacteriostatic water vial and draw out the required volume for reconstitution—commonly 2ml for a 10mg vial.
Add Bacteriostatic Water to Peptide:
- Slowly inject the bacteriostatic water into the peptide vial, aiming for the side wall to minimize direct contact with the peptide powder.
- After adding solvent, gently swirl or roll the vial until the powder is completely dissolved (never shake vigorously).
Storage:
- Store reconstituted peptides in the refrigerator.
- Avoid freezing and keep out of direct sunlight.
Dosing Calculation Example for a 10mg Vial
If you add 2ml of bacteriostatic water to a 10mg peptide vial:
Concentration Calculation:
- Total peptide = 10mg
- Total solvent = 2ml
- Concentration
Finding a 1mg Dose:
- Desired dose = 1mg
- Volume to draw = or 20 units on an insulin syringe
- Simply multiply 0.2ml by your desired mg dose to calculate how much to inject, or follow the chart at the end of this article.
Tips for Accuracy and Safety
- Always use sterile techniques and tools to prevent contamination.
- Double-check calculations before dosing.
- Label vials with concentration and date to avoid mix-ups.
- Store reconstituted peptides correctly for prolonged stability.
Summary Table: Example Dosages (2ml Bacteriostatic Water Added to 10mg vial)
| Dose (mcg or mg) | Concentration (mg/ml) | Volume to Withdraw (ml) | Insulin Syringe Units (U100 syringe, 1ml=100 units) |
|---|---|---|---|
| 250 mcg (0.25 mg) | 5 mg/ml | 0.05 ml | 5 units |
| 500 mcg (0.5 mg) | 5 mg/ml | 0.10 ml | 10 units |
| 1 mg | 5 mg/ml | 0.20 ml | 20 units |
| 2 mg | 5 mg/ml | 0.40 ml | 40 units |
| 4 mg | 5 mg/ml | 0.80 ml | 80 units |
| 6 mg | 5 mg/ml | 1.20 ml | 120 units* (exceeds 1ml syringe capacity) |
| 8 mg | 5 mg/ml | 1.60 ml | 160 units* (exceeds 1ml syringe capacity) |
| 10 mg | 5 mg/ml | 2.00 ml | 200 units* (exceeds 1ml syringe capacity) |
Reconstituting peptides is easy, but attention to sterile technique, accurate dosing, and proper storage are key to preserving peptide quality and research outcomes.
